Fig. 3From: LcMYB4, an unknown function transcription factor gene from sheepgrass, as a positive regulator of chilling and freezing tolerance in transgenic ArabidopsisSubcellular localization and transactivation assay of LcMYB4. a Subcellular localization of LcMYB4 in tobacco (Nicotiana benthamiana) leaf epidermal cells. 35S:GFP and 35S:LcMYB4 plasmids were transformed into tobacco leaf epidermal cells, and GFP fluorescence was visualized under a confocal laser-scanning microscope. From left to right, GFP fluorescence, bright-field, and overlays of the GFP fluorescence and bright-field. Bar = 50 μm. b Transactivation assay of LcMYB4. E, Experimental group (pBridge-LcMYB4) P, Positive control (pBridge-LcWRKY5); N, Negative control (pBridge)Back to article page