Fig. 7From: Over-expression of a γ-tocopherol methyltransferase gene in vitamin E pathway confers PEG-simulated drought tolerance in alfalfaRelative expression of drought stress-related genes and ABA content in transgenic and control plants. MsActin (EU664318) and MsADF (JZ818469) were used as the reference genes to normalize the gene expression and showed similar results. The 2-ΔΔCTT method was used for qRT-PCR analysis with three biological replicates. Values are the mean ± SEM. Significant differences: *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001Back to article page