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Fig. 5 | BMC Plant Biology

Fig. 5

From: Over-expression of a γ-tocopherol methyltransferase gene in vitamin E pathway confers PEG-simulated drought tolerance in alfalfa

Fig. 5

Overexpression of the MsTMT gene improved water use efficiency in alfalfa. a. Relative chlorophyll content of mature leaflets. b-e. CO2 assimilation rate, transpiration rate, transpiration efficiency and stomatal conductance were estimated from gas exchange measurements on mature leaflets using the LI-COR 6800 system. f. Temperature of mature leaflets was obtained with the chlorophyll analyzer. The first fully expanded leaf from top of branch of each plant was selected to analysis in those measurements. g. Analysis of leaf carbon isotope composition (δ) of transgenic alfalfa. The first fully expanded leaf from top of branch was collected for analysis. Values are the mean ± SEM. Significant differences: *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001

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