Fig. 4

Comparison of physiological indexes of transgenic lines and control plants under stress condition. a. Water loss rates were determined at 0, 0.5, 1, 2, 3, 4, 5 and 6 h. b. Water content of leaves. c-d. Histochemical detection of H₂O₂ in leaves of control and transgenic lines after water loss at indicated times. Scale bar = 0.5 cm. Quantification of DAB staining after 72 h PEG treatment with Image j. e-h. Relative electrolyte leakage, MDA content, and soluble sugar and proline content of mature leaves under stress. Each value indicates the mean ± SEM. Significant differences: *P ≤ 0.05, **P ≤ 0.01