Table 4 Primers used for gene cloning and qRT-PCR
Reaction | Gene ID | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|---|
Genes cloning | GENE_011842 | ATGGCACGACTCTCTCTCAG | TCAGAACTGGTTCATCGGCGGT |
GENE_013439 | ATGTACTGTGTGTATGGCCT | CTACGGTTCATAACCTAGAGCATCC | |
GENE_014314 | ATGGTGCATGGCTTGGAGTG | CTATAGCTCATAACCTAGAG | |
qRT-PCR | GENE_011842 | GAAACAAAACGGGGTGGACG | GGTCCGTGGTTGCTACTGAA |
GENE_021484 | GCTGATAGTGGTGGACTCGG | CATGTCGTTGTGGTCGGAGA | |
GENE_005471 | ACAAGACCGGATCGACAACC | ACTTCTGGCTCTCTTGCGTC | |
XLOC_024652 | CAAGGGAAGGTGCAACAACG | AGATGTTCGCCTTCGGGTTT | |
GENE_014314 | TGAAGTCCATTCGCCTCACC | TGTTGTCAGCTTTGACCCGA | |
GENE_015734 | GCACTATCACATCGGCACCT | GGCTCTCGGAAGATGGTGAC | |
XLOC_016866 | GCGATTTCGAGACCAGGGAT | GTAGCTCTTGGTGCTCGGTT | |
GENE_017041 | GCTGACGACGGAGGAGTTAG | CCGTGCAATCCTCAGCCATA | |
GENE_026033 | CACCACACCAGAGCATCCTT | CGCAGCCTTGTAATCGAAGC | |
GENE_009777 | GCAATCTTGCTTCTGCGACC | CAATCTCAACACCAACGCCC | |
XLOC_011209 | TACCTGGCGAAACCACGAAA | TTCATCAAACCGCTCCGTCA |