Table 2 Transformation efficiency of the one-step ARM transformation method for different soybean ecotypes
Genotype | Numbers of plants infected | Numbers of the explants produced hairy roots | Numbers of hairy roots (per seedling) | Number of GUS-positive roots (per seedling) | Transformation efficiency | Transformation frequency |
|---|---|---|---|---|---|---|
Williams82 | 30 | 30 | 12.4 ± 3.8 A | 6.5 ± 2.7 A | 100% | 52.4% |
Jindou 17 | 30 | 30 | 8.9 ± 2.0 CD | 3.4 ± 0.9 C | 96.7% | 38.2% |
Zhonghuang 39 | 32 | 32 | 8.7 ± 2.0 D | 3.1 ± 0.9 C | 96.7% | 35.6% |
Xiangfeng4 | 32 | 32 | 10.6 ± 2.2 BC | 3.8 ± 1.1 C | 100% | 35.8% |
Hedou19 | 30 | 30 | 10.0 ± 2.3 BCD | 3.9 ± 1.1 C | 100% | 39% |
PI377578 | 30 | 30 | 8.9 ± 2.1 CD | 3.1 ± 1.2 C | 96.7% | 34.8% |
Peking | 30 | 30 | 11.5 ± 2.4 AB | 5.1 ± 1.3 B | 100% | 44.3% |