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Table 1 Monosaccharide composition analysis of AGPs extracted from aerial part of 40-day-old glcat CRISPR mutants and WT (Col-0)*

From: Elucidating the roles of three β-glucuronosyltransferases (GLCATs) acting on arabinogalactan-proteins using a CRISPR-Cas9 multiplexing approach in Arabidopsis

  Fuc Rha Ara Gal Glu Xyl Man GalA GlcA
WT 2.8 ± 0.7 4.8 ± 1.0 26.3 ± 2.4 39.1 ± 0.9 4.2 ± 0.3 7.0 ± 1.4 3.1 ± 0.6 5.0 ± 0.8 6.0 ± 1.3
glcat14b 2.7 ± 0.4 5.1 ± 0.6 24.6 ± 0.9 38.2 ± 0.4 3.7 ± 2.2 8.5 ± 1.2 3.3 ± 0.6 7.6 ± 1.4 6.1 ± 0.9
glcat14c 2.1 ± 0.4 4.4 ± 0.7 25.7 ± 2.1 41.5 ± 0.1 4.5 ± 0.2 6.7 ± 1.0 2.8 ± 0.4 7.2 ± 0.5 5.4 ± 0.9
glcat14a glcat14b 2.2 ± 0.2 3.4 ± 0.5 30.5 ± 3.8 42.3 ± 1.0 3.2 ± 1.0 5.6 ± 0.4 2.8 ± 0.2 4.5 ± 0.0 4.4 ± 0.4
glcat14b glcat14c 2.4 ± 0.2 4.1 ± 1.0 27.5 ± 0.4 44.7 ± 0.4 4.1 ± 0.1 7.0 ± 0.2 2.8 ± 0.0 3.8 ± 0.2 5.3 ± 0.4
glcat14a glcat14b glcat14c 2.8 ± 0.4 4.0 ± 1.2 30.7 ± 1.6 41.2 ± 1.0 3.6 ± 0.3 7.0 ± 0.7 2.7 ± 0.5 4.1 ± 1.3 3.6 ± 1.2
  1. *Values are relative to total sugar composition (expressed as mol %) of triplicate assays ± SE
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BMC Plant Biology

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