Fig. 4

The phenotypes of transgenic lines with ectopic expression of OsGRF1. a The expression levels of OsGRF1 in 2-week-old seedlings of the non-transformed plants (NT) and OsGRF1RNAi. Expression was analyzed by qRT-PCR. **, Significant difference at P < 0.01 compared with expression in the non-transformed plants (NT) by Student’s t-test (n = 3; means ± SDs). b The phenotype of the complete leaf from second to fourth position in the 3-week-old seedlings of the three backgrounds of OsGRF1 (WT, mOsGRF1OE, OsGRF1RNAi). Bar = 10 cm. c The growth curve of the biomass of the suspension-cultured cells in the three backgrounds of OsGIF1. The suspension-cultured cells were harvested, dried, and weighed at given time. DW: dry weights. The data were the means of three biological repetition ± SE. d The morphology and size of the cells in suspension-cultured system originated from leaf calli in the three backgrounds of OsGRF1. Bar = 50 μm. e The expression levels of cycOs1 and cycOs2 in the leaves of three backgrounds of OsGRF1. Expression was measured by qRT-PCR. **, Significant difference at P < 0.01 compared with expression in the non-transformed plants (NT) by Student’s t-test (n = 3; means ± SDs)