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Fig. 3 | BMC Plant Biology

Fig. 3

From: Coordination between GROWTH-REGULATING FACTOR1 and GRF-INTERACTING FACTOR1 plays a key role in regulating leaf growth in rice

Fig. 3

The Phenotypes of the non-transformed rice, OsGRF1OE, and mOsGRF1OE.a The regions complementary to OsmiR396 in OsGRF1 and mOsGRF1 (mutated OsGRF1) mRNA and the corresponding amino acid sequence. The mutated sites (shown in blue) in mOsGRF1 were artificially brought into to reduce the degree of the complementarity to OsmiR396 without alteration of amino acid sequence. b The RNA levels of OsmiR396 and OsGRF1 in the seedlings of the non-transformed plants (NT), OsGRF1OE, and mOsGRF1OE. OsmiR396 was detected by northern blot and U6 was served as loading control. OsGRF1 was analyzed by qRT-PCR, **, Significant difference at P < 0.01 compared with expression in the non-transformed plants (NT) by Student’s t-test (n = 3; means ± SDs). c The length of different leaves in 4-week-old seedlings of the non-transformed plants (NT), OsGRF1OE, and mOsGRF1OE. *, Significant difference at P < 0.05 compared with the non-transformed plants (NT) by Student’s t-test (n = 5; means ± SDs). d The morphology of the incomplete leaf in 4-week-old seedlings of the non-transformed plants (NT), OsGRF1OE, and mOsGRF1OE

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