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Fig. 1 | BMC Plant Biology

Fig. 1

From: Coordination between GROWTH-REGULATING FACTOR1 and GRF-INTERACTING FACTOR1 plays a key role in regulating leaf growth in rice

Fig. 1

The expression patterns of OsGRF1 and OsGIF1 in rice. a The expression levels of OsGRF1 and OsGIF1 in flowers (adult plants) and different tissues of the 4-week-old seedlings in non-transformed rice (NT). Expression was analyzed by qRT-PCR. *, Significant difference at P < 0.05, **, Significant difference at P < 0.01 compared with expression in Older Stems by Student’s t-test (n = 3; means ± SDs). OS: Older Stem, basal internodes in stem of 4-week-old-seedlings; YS: Younger Stem, 5-mm-long shoot tips containing SAM (Shoot Apical Meristem) of 4-week-old-seedlings; OL: Older Leaf, the leaves in basal shoot of 4-week-old seedlings; YL: Younger Leaf, leaf tips and leaf primordium of 4-week-old-seedlings; F: Flowers in adult stage; OR: Older Root, the basal region in roots of 4-week-old seedlings; YR: Younger Root, 5-mm-long root tips of 4-week-old seedlings. b The RNA levels of OsGRF1 and OsGIF1 analyzed by northern blot in different tissues of the non-transformed rice (NT). Total RNA from 1-week-old-seedlings (lane SE) and different tissues as described in (A), including older stem (lane OS), Younger stem containing SAM (Shoot Apical Meristem) (lane YS), older leaf (lane OL), younger leaf (lane YL), the flowers in adult stage (lane F), older root (lane OR), and younger root (lane YR) was loaded and electrophoresed. Then the electrophoretic products were transferred and probed by labeled anti-sense sequences. The rRNA bands were visualized by ethidium bromide staining and served as loading control. c The protein levels of OsGIF1 and OsGRF1 analyzed by western blot. Total protein extracted from 2-week-old seedlings of the non-transformed plants (NT) was immunoblotted by anti-OsGIF1 (the left) and anti-OsGRF1 (the right) respectively. Actin immunoblotted by anti-Actin was served as control

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