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Fig. 4 | BMC Plant Biology

Fig. 4

From: Genome-wide identification and characterisation of Aquaporins in Nicotiana tabacum and their relationships with other Solanaceae species

Fig. 4

In planta sub-cellular localisation of PIP, TIP and NIP aquaporins. Confocal images of root cortical cells of transgenic 8-day-old Arabidopsis seedlings. a, b, d, f GFP marker lines; false coloured purple. c, e, g NtAQP:GFP lines; false coloured green. Subpanels (i-iv) are; (i.) Optical cross-section midway through a root cortical cell. (ii) GFP signal associated with nucleus; confocal image (left) DIC image (right). (iii.) Close-up of cell peripheral margin. (iv.) Maximum intensity projections compiled from serial z-stack images. a GFP-only localisation. b Plasma membrane (PM:GFP) marker. cNtPIP2;5 t (PIP:GFP). d Endoplasmic reticulum (ER:GFP) marker. The ER is known not uniformly be present around the cell periphery which is reflected by regions of bright GFP signal (solid arrowhead) interspersed with regions of no GFP signal (open arrowhead). eNtNIP2;1 s (NIP:GFP). f Tonoplast (Tono:GFP) marker showing characteristic features of the tonoplast membrane including, transvacuolar strands (v) and general undulating appearance (arrow). gNtTIP1;1 s (TIP:GFP). Notable sub-cellular features are marked by a; asterisks for the nucleus, ‘V’ for transvacuolar strands, arrowheads indicate instances of varied brightness (solid = high signal, empty = no signal) in GFP fluorescence in d (iii) and e (iii), or undulations of the tonoplast in f (iv) and g (iv). Scale bar 5 μm

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