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Table 5 PCR primers used for species identification, molecular characterization, and gene expression

From: Diversity of nitrogen-fixing rhizobacteria associated with sugarcane: a comprehensive study of plant-microbe interactions for growth enhancement in Saccharum spp.

Target Gene

Primer

Name

Nucleotide Sequence (5′ ------- → 3′)

Product Size (bp)

Reference

16S

PA-F

PH-R

AGAGTTTGATCCTGGCTCAG

AAGGAGGTGATCCAGCCGCA

1300–1500

[95]

rpoB

RPO-F

RPO-R

ATCGAAACGCCTGAAGGTCCAAACAT

ACACCCTTGTTACCGTGACGACC

> 1100

[94]

BOX

BOX A1R

CTACGGCAAGGCGACGCTGACG

50–5000

[96]

ERIC

ERIC-1

ERIC-2

ATGTAAGCTCCTGGGGATTCAC

AAGTAAGTGACTGGGGTGAGCG

50–5000

RTq-PCR Primers

NifH

Pol-F

Pol-R

TGCGAYCC-SAARGCBGACTC

ATSGCCATCATYTCRCCGGA

RTq-PCR

[97]

 Glyceraldehyde 3-phosphate dehydrogenase

GAPDH-1

GAPDH-2

CTCTGCCCCAAGCAAAGATG

TGTTGTGCAGCTAGCATTG

RTq-PCR

[98]

SuPAL

PAL-F

PAL-R

CTCGAGGAGAACATCAAGAC

GTGATGAGCTCCTTCTCG

RTq-PCR

[74]

SuCAT

CAT-F

CAT-R

CTTGTCTGGAGCACATACACTTGGA

TTCTCCGCATAGACCTTGAACTTTG

RTq-PCR

[72]

SuSOD

SOD-F

SOD-R

TTTGTCCAAGAGGGAGATGG

CTTCTCCAGCGGTGACATTT

RTq-PCR

[75]

ScChi

ScChi-QF

ScChi-QR

ACGGCTACGGCGACAACA

GTCCGCTGACCAGATGAAGAG

RTq-PCR

[71]

ScGluD1

D-QF

D-QR

TGCTACTTCTTATCCACCCTCTG

CGTTGACATAGAAAGGTGAGCC

RTq-PCR

[99]