Fig. 5
Protein-protein interaction of TaOAT with other wheat proteins. a The interacting protein partners predited by STRING database. The TaOAT-5BL (Traes_5BL_1D3F1BCC2.1) amino acid sequence was used to search for its interacting genes in wheat. Nodes represent the single protein coding locus and edges represent the meaningful interaction that shared the same functions. Color nodes represent the first shell of interactors while white nodes represent the second shell of interactors. Empty nodes represent the protein of unknown 3D structure while filled nodes represent the known or predicted 3D structure. b Confirmation of the predicted interaction results by the yeast two hybrid. The figure on the left hand side represents the yeast growth on two absent (Leu/Trp) medium. The figure on the right hand side represents the yeast growth on three absent (Leu/Trp/His) medium. The specific interaction was determined by the growth of transformants on three absent (Leu/Trp/His) medium. 100 represent the original culture sample. 10− 1–10− 3 showed 10 fold serial dilution of the sample