Fig. 3

Determination of chromosomal location of TaOAT genes in hexaploid wheat using specific primers and nullitetrasomic lines. The absence of the band 864 bp in size in lane 1 shows that the primer is specific to TaOAT-5AL due to the absence of chromosome 5A in N5A/T5B. Similarly, the absence of the bands 882 and 840 bp in size in lane 2 and 3 shows the specificity of the primers to TaOAT-5BL and TaOAT-5DL as chromosomes 5B and 5D are absent in N5B/T5A and N5D/T5A lines, respectively. M: DL2000 DNA ladder (TianGen Biotech. Beijing Co., Ltd.); 1–4: Chinese Spring chromosome group 5 nullitetrasomic lines where N represents the null chromosome, and T represents the tetra chromosome in which 2 chromosomes replaced or shuffled the respective absent two chromosomes (1: N5A/T5B, 2: N5B/T5A, 3: N5D/T5A, and 4: Chinese Spring)