Fig. 6

Validation of RNA-seq results by RT-qPCR. a Transcript levels of 16 genes in the three samples were detected by RT-PCR. Light grey bars indicate transcript abundance changes estimated from RNA-seq. Dark grey bars with associated standard deviations represent the normalized relative expression levels determined by real-time quantitative PCR using the 2^-△△CT method. b Correlation of gene expression levels between RNA-seq and RT-qPCR data