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Fig. 8 | BMC Plant Biology

Fig. 8

From: MYB transcription factor PdMYB118 directly interacts with bHLH transcription factor PdTT8 to regulate wound-induced anthocyanin biosynthesis in poplar

Fig. 8

JAZ1 protein content and ABG expressions in the wounded leaves of transgenic poplar plants. PtrCHS1, PtrCHI1, PtrF3H, PtrF3’H, PtrF3’5’H, PtrDFR2 and PtrANS1 are the ABG genes respectively encoding chalcone synthase, chalcone isomerase, flavanone 3-hydroxylase, flavanone 3′-hydroxylase, flavonoid 3′5’-hydroxylase, dihydroflavonol 4-reductase and anthocyanidin synthase in Populus. a Western blotting analysis of JAZ1 contents. Total proteins were extracted from the untreated or treated leaves of PdMYB118 overexpressing plants. JAZ1 antibodies (JAZ1) and plant actin antibodies (Actin) were used for the hybridization. The original uncropped blot image was shown in additional file 7: Fig. S7. b qRT-PCR analyses of ABGs in the untreated or treated leaves of wild type and PdMYB118 overexpressing plants. The leaves were mechanically wounded by leaf-disc method for 2 days. Then RNA was extracted for qRT-PCR analyses. Gene expression level in wide type leaves was set to 1. Values are means and standard deviations of three biological replicates (n = 3). ** and ***, significant differences at P < 0.01 and P < 0.001 (Student’s t-test). WT, leaves of wide type poplar plant; L2 and L7, leaves of transgenic plants overexpressing PdMYB118; WT-W, wounded leaves of wide type poplar; L2-W and L7-W, wounded leaves of transgenic plants

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