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Fig. 2 | BMC Plant Biology

Fig. 2

From: MYB transcription factor PdMYB118 directly interacts with bHLH transcription factor PdTT8 to regulate wound-induced anthocyanin biosynthesis in poplar

Fig. 2

qRT-PCR analyses of PdMYB118 and ABGs in the wounded leaves of wild type poplar plants. The mature leaves of poplar plants grown in greenhouse were treated using leaf-disc method for various time intervals for RNA extraction. Gene expression in the untreated leaves (0 h) was set to 1. PtrMYB118 and PtrTT8 are respectively the homology of PdMYB118 and PdTT8, and PtrCHS1, PtrCHI1, PtrF3H, PtrF3’H, PtrF3’5’H, PtrDFR2 and PtrANS1 are the ABG genes respectively encoding chalcone synthase, chalcone isomerase, flavanone 3-hydroxylase, flavanone 3′-hydroxylase, flavonoid 3′5’-hydroxylase, dihydroflavonol 4-reductase and anthocyanidin synthase in Populus. Three independent replicates of measurements were performed for each time point, and the values are means and standard deviations (n = 3). ** and ***, significant differences at P < 0.01 and P < 0.001 (Student’s t-test)

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