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Fig. 2 | BMC Plant Biology

Fig. 2

From: A modular cloning toolkit for genome editing in plants

Fig. 2

Assembly of level 1 sgRNA expression modules to be used with respective Cas9 nucleases. During the first step (a) annealed complementary oligos encoding the guide are inserted into a level 0 acceptor with the sgRNA backbone using BpiI. During the second step (b), sgRNA is fused with the respective Pol III promoter using BsaI

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