Fig. 6

ZNC enhanced RNA silencing. aN. benthamiana leaves were inoculated with Agrobacterium harboring PBI121-GFP after water (up panel) or 150 ng/mL of ZNC (down panel) treatment for 2 h. Then, the leaves were examined under UV light at 3 dpi. b The relative expression level of GFP was detected by qRT-PCR. Data are shown as the mean (n = 3) ± SD, and ** indicates extremely significant differences determined using the Student’s t-test (p < 0.01). c Northern blot analysis of GFP siRNA extracted at 3 dpi from patches, the cropped gel and blot images were shown in Fig. 6c, and tRNA was used as loading control for siRNA. d The relative expression of genes related to RNA silencing pathway was detected using qRT-PCR after ZNC treatment for 2 h. Data are shown as the mean (n = 3) ± SD. * indicates significant differences determined using the Student’s t-test (p < 0.05), and ** indicates extremely significant differences determined using the Student’s t-test (p < 0.01).