Fig. 3

IbSUT4 function analysis. a, b Expression of IbSUT4 in the SUSY7/ura3 yeast strain grown on sucrose or on glucose as the sole carbon source. The plates were incubated at 30 °C for 3 days before observation. A. SUSY7/ura3 yeast transformed with the empty P416 vector and with IbSUT4 in the P416 vector, and grown on 2% sucrose. B. SUSY7/ura3 yeast transformed with the empty P416 vector and with IbSUT4 in the P416 vector, and grown on 2% glucose. c, dIbSUT4 shows sucrose uptake activity in Arabidopsis thaliana. C. RT-PCR analysis of IbSUT4 expression in the WT and three transgenic lines (SUT4–1, SUT4–7, and SUT4–10). D. Uptake of fluorescent esculin in seven-day-old seedlings. a, b, c The blots/gels are cropped from Additional file 2, Fig. S.2,3. Scale bar = 300 μm