Table 3 Primers used for amplification and complete sequencing of the GBSSI (granule-bound starch synthase I) gene in waxy and non-waxy cassava genotypes
From: Identification and validation of mutation points associated with waxy phenotype in cassava
Primer | Sequence | Expected fragment size (bp) |
|---|---|---|
MeGBSSIpA-F | TGGCGAAGTCCCACCATTAC | 974 |
MeGBSSIpA-R | TGTACTGGTCATAGCGGGGA | |
MeGBSSIpB-F | TCCCCGCTATGACCAGTACA | 988 |
MeGBSSIpB-R | ACAAGTCACCAACCCCGAAA | |
MeGBSSIpC-F | CACTGCTCTGCTTCCATGTTATCT | 936 |
MeGBSSIpC-R | TCTCACAACACAACCAAGGACATC | |
MeGBSSIpD-F | CAGAAGTCGGATTGCCTGTTGATA | 931 |
MeGBSSIpD-R | GCTACCAGTCAATCCAATTTGCAC | |
MeGBSSIpE-F | TGACTAAGTATCTAGGAGGCTCA | 1038 |
MeGBSSIpE-R | GAAGGGAAGAAAGAAACTGAATGAC |