Table 1 Characteristics of 16 microsatellite primers developed for Angelica dahurica
Locus | Forward and reverse primer sequences | Motifs | product Size range (bp) | Ta | GenBank Accession No. |
|---|---|---|---|---|---|
AD1 | F:TCCTCCAGCTGGCATAATAATAA R:ATTAAAAAGAACAAGGGGCTCAA | (TGC)6 | 111–123 | 55 | MH220032 |
AD2 | F:CTTTTTCCCCAATTCCTATCAAC R:CACACCCGTAGAGATACACACTG | (TA)9 | 136–188 | 55 | MH220033 |
AD4 | F:TTAACAACTGGCTTTGATCCATT R:TGGAGGTTTGTACGATTTTATGG | (TG)9 | 90–126 | 50 | MH220034 |
AD7 | F:GCTCTCTTAAATTTCACCCCAAC R:TACTAGATTCTTCCAGAGCGACG | (ATTACC)4 | 131–155 | 55 | MH220035 |
AD8 | F:TTCAACATGGTCATGTGAGTGAT R:CCGTTGGAGGTCTTCTTGTAAAT | (GGAGTG)4 | 140–164 | 53 | MH220036 |
AD9 | F:CAACACACATGATCCAGAAGAAA R:GAGCTGGAGATAGTCTGTTGCAT | (TCTGCA)10 | 99–159 | 50 | MH220037 |
AD10 | F:AGACTGCACCTGTCTCATTTTTC R:GGCTTGTAATTAATCTTTGCACC | (GT)9 | 116–140 | 50 | MH220038 |
AD11 | F:TTCGTCATTTAGAAACGATAGCA R:TCAATGGATACCACCACATCATA | (TCT)7 | 127–142 | 50 | MH220039 |
AD12 | F:AAGTTCAATATTCCCCTGGTGAC R:GTACATTATACCCTCCCCAAACC | (AAACC)4 | 126–131 | 55 | MH220040 |
AD14 | F:TGTACTCCATGGACTGGAGTCTT R:TTTGTTTTCTGACAAAGCCAAAT | (TCA)7 | 108–123 | 50 | MH220041 |
AD17 | F:GGATCATGTTGATGATGGAAAAT R:TTCGATTACTACAGCAGATGAGC | (AGA)7 | 145–163 | 50 | MH220042 |
AD19 | F:CCCCATTTCTCCCATAGATAGAT R:CCATTAATTGTTCTGCATTTTCC | (GCA)6 | 125–140 | 53 | MH220043 |
AD22 | F: AAACAATATCAAATCAAATGGCGR: GTGGTGATGATGAATCTTGTGAA | (TTC)6 | 84–99 | 50 | MH844986 |
AD23 | F: GCTTGACATATATCATCGCCTTTR:TAGACCAAGAGCCAAATAAACCA | (CTT)6 | 130–142 | 50 | MH844987 |
AD24 | F: GCGAGATGGAAATGACAAATTCTR: ATCCCACCATTTCCTCATTAAGT | (GCA)6 | 108–117 | 50 | MH844988 |
AD26 | F: ACAAACAAACCAAATCAATCTCGR: TTATCTTCCAATTGCTTGTTGCT | (CAAAC)4 | 118–123 | 50 | MH844989 |