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Fig. 5 | BMC Plant Biology

Fig. 5

From: Integration of sRNA, degradome, transcriptome analysis and functional investigation reveals gma-miR398c negatively regulates drought tolerance via GmCSDs and GmCCS in transgenic Arabidopsis and soybean

Fig. 5

Responses of transgenic plants to simulated drought conditions. a, b Expression of the gma-miR398c precursor (gma-MIR398c) and the relative expression of ath-miR398c target genes in transgenic Arabidopsis lines determined by RT-qPCR. Expression levels were normalized against that of the Actin gene. c Seeds of WT, OE-vector and OE-miR398c were germinated on plates containing different concentrations of mannitol. Germination rates were analyzed after 14 days. d Survival rate of Arabidopsis seedlings after 10 days cultivation on 300 mM D-mannitol medium. Vertical bars represent the standard deviation of the means (n = 3, 200 seeds per replicate). Asterisks indicate significant differences between the transgenic and WT lines (*, P < 0.05; **, P < 0.01; ***, P < 0.001)

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