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Fig. 8 | BMC Plant Biology

Fig. 8

From: Genome-wide identification of the amino acid permease genes and molecular characterization of their transcriptional responses to various nutrient stresses in allotetraploid rapeseed

Fig. 8

The qRT-PCR-assisted transcriptional characterization of the amino acid permease (AAP) genes in Brassica napus under different nitrogen (N) form conditions. Differential expression of the BnaAAP1 (a), BnaAAP2 (b), BnaAAP3 (c), BnaAAP4 (d), BnaAAP5 (e), BnaAAP6 (f), BnaAAP7 (g) and BnaAAP8 (h) genes under nitrate (NO3) and ammonium (NH4+) conditions. For the transcriptional analysis, the rapeseed seedlings were first hydroponically cultivated under 6.0 mM nitrate (NO3) for 10 d, and then were transferred to an N-free solution for 3 d. Subsequently, the above seedlings were sampled after treatment with 6.0 mM ammonium (NH4+) for 3 d. The shoots and roots were individually sampled, and each sample includes three independent biological replicates. The significance level of P < 0.05 is used as the threshold to identify the differential BnaAAP expression under high and low nitrate treatments. The differentially expressed genes with higher expression between different treatments in the shoots or roots are indicated with asterisks

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