Table 2 Wheat SSR and EST-STS markers used in this study to analyze the chromosomal composition of TR77
markers | locus | 7182 | Trs-372 | TR77 | P | Primer (5′-3′) | Tm (°C) |
|---|---|---|---|---|---|---|---|
Barc130 | 5DS | + | – | – | F: CGGCTAGTAGTTGGAGTGTTGG R: ACCGCCTCTAGTTATTGCTCTC | 50 | |
Xcfd189 | 5DS | + | – | – | F: GCTAAAGCCACATAGGACGG R: GCACAAGATTTTGCAAGGCT | 60 | |
Xwmc318 | 5DL | + | – | – | F: CGTAAAATTACGGTGCATTGAT R: GTGGACTTTTGTGGTTTTTGAG | 60 | |
Xgdm153 | 5DL | + | – | – | F: TATAGGCAAATTAATTAAGACG R: ATCTTTATGTGAGTACACTGC | 50 | |
Xcfd8 | 5DL | + | – | + | F: ACCACCGTCATGTCACTGAG R: GTGAAGACGACAAGACGCAA | 60 | |
Xgwm182 | 5DL | + | – | + | F: TGATGTAGTGAGCCCATAGGC R: TTGCACACAGCCAAATAAGG | 60 | |
Xwmc357 | 5DL | + | – | + | F: TAGTGGGTGACCGGTCAAGA R: TGGACGGATTTGGTCATTTC | 60 | |
BF146187 | 5AS 5BS 5DS | – | – | + | + | F: CAAGGTGCAACAGTTCATGG R: GGTCACAGAAATATGCGGCT | 60 |
BE444644 | 5AS 5BS 5DS | – | – | + | + | F: AAGCTTGCTGAGCTTTCTGG R: TTGAGGGATGTAGGGCAAAG | 60 |
CD452608 | 5AS 5BS 5DS | – | – | + | + | F: TGATGTCTTGTCGTGGTCGT R: TTTTGGATGCAGCAAGACAG | 60 |