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Fig. 1 | BMC Plant Biology

Fig. 1

From: Molecular cytogenetic characterization of a novel wheat–Psathyrostachys huashanica Keng T3DS-5NsL•5NsS and T5DL-3DS•3DL dual translocation line with powdery mildew resistance

Fig. 1

Cytological and genomic in situ hybridization (GISH) analyses of root tip cells from line TR77. a Mitotic metaphase, 2n = 42. b GISH analysis of TR77. GISH was conducted using the total DNA from P. huashanica as the probe and 7182 as the block. Two chromosomal segments with fluorescent hybridization signals (yellow-green) were detected as alien chromosomes in the mitotic metaphase. c Enlarged a pair of the translocated chromosomes. The alien chromosome segment consists of the short arm and a small portion of the long arm attached to the centromere. Chromosomes were counterstained with Propidium Iodide (red)

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