Fig. 2

Gene expression analyses of responsive ROS scavenging genes. a Differentially expressed genes obtained by RNA-Seq were illustrated in relation to the major pathways they were involved. Colored columns corresponded to fold changes of the transcripts as indicated by the definition of color gradients (bottom). The symbol ‘+’ and the red gradient columns represented up-regulated genes and the fold of induction; while ‘-‘and the green gradient columns referred to down-regulated genes. b The qRT-PCR validation of randomly selected genes coding for ROS scavenging systems. The relative expression levels were normalized against OsActin. Values indicated were means of three independent replicates. Numbered responsive genes were annotated as followings: 1. Os01g0949700, putative glutathione S-transferase; 2. Os03g0785900, probable glutathione S-transferase GSTU1; 3. Os01g0369700, putative glutathione transferase 4; 4. Os01g0949800, putative glutathione S-transferase; 5. Os01g0949750, putative glutathione S-transferase; 6. Os10g0365200, glutathione S-transferase; 7. Os10g0527800, glutathione S-transferase OsGSTU12; 8. Os10g0481300, glutathione S-transferase; 9. Os10g0525500, glutathione S-transferase parC; 10. Os01g0372400, putative glutathione S-transferase; 11. Os10g0528300, glutathione S-transferase OsGSTU4; 12. Os10g0415300, glutathione reductase; 13. Os08g0557600, monodehydroascorbate reductase; 14. Os07g0638400, 1-Cys peroxiredoxin B; 15. Os05g0499300, peroxidase 1; 16. Os07g0677300, peroxidase 2; 17. Os05g0134800, Class III peroxidase 67; 18. Os02g0236600, Class III peroxidase 27; 19. Os03g0234900, Class III peroxidase 39; 20. Os03g0368000, Class III peroxidase 42; 21. Os06g0695300, Class III peroxidase 92; 22. Os07g0564500, NADH dehydrogenase [EC:1.6.99.3]