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Fig. 4 | BMC Plant Biology

Fig. 4

From: Overexpression of an apple LysM-containing protein gene, MdCERK1–2, confers improved resistance to the pathogenic fungus, Alternaria alternata, in Nicotiana benthamiana

Fig. 4

MdCERK1–2 OE improved the resistance of Nb plants to A. alternata. a Transgenic plants were screened using qRT-PCR. Numbers represent different lines. b Positive transgenic plants were verified using immunobloting with an anti-HA antibody. c Representative leaves with lesions resulted from inoculation with fungal discs of A. alternata (0.5 cm in diameter). Photos were taken at 8 and 10 dpi. d Quantitation of the lesions was conducted by measuring lesion diameter. The data were analyzed for statistical differences by a two-way ANOVA followed by Tukey’s post-hoc test. Bars indicate the mean ± SD (n = 5). Asterisks indicate significant differences when compared to the WT (** p < 0.01)

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