Table 1 Parameters in the site analysis, branch and branch-site analyses of NADP-ME-A

Site analysis

–

M0:(one-ratio)

0.091

ω = 0.091

–

–

–

–

M3:(discrete)

0.117

p0 = 0.601 ω0 = 0.019

4

0.000(M0 vs M3)

–

p1 = 0.360 ω1 = 0.175

p2 = 0.039 ω2 = 1.091

Branch analysis

–

M0

–

ω = 0.091

–

–

–

–

a1

–

ω₀ = 0.092 ω₁ = 0.006

1

< 0.001

–

–

a2

–

ω₀ = 0.092 ω₁ = 0.016

1

< 0.001

–

–

a3

–

ω₀ = 0.092 ω₁ = 0.018

1

< 0.001

–

–

a4

–

ω₀ = 0.090 ω₁ = 0.314

1

0.095

–

–

a5

–

ω₀ = 0.091 ω₁ = 0.085

1

0.827

–

–

a6

–

ω₀ = 0.090 ω₁ = 0.358

1

0.218

–

–

a7

–

ω₀ = 0.090 ω₁ = 0.158

1

0.121

–

Branch-site analysis

–

M1

–

p₀ = 0.915 p₁ = 0.085

–

–

–

a1

Model A

–

p0 = 0.871 p1 = 0.079(p_2a + p_2b = 0.050)

2

0.0002 (ModelA vs M1)

Prob > 0.6 (178 M, 188Q, 193D, 326E, 330 L, 344 V, 345S, 375 L)

ω2 = 37.078

a2

Model A

–

p₀ = 0.897 p₁ = 0.081 (p_2a + p_2b = 0.002)

2

0.0520 (ModelA vs M1)

Prob > 0.6 (11 L, 63D^a, 94H, 110R, 344 V)

ω2 = 8.513

a3

Model A

–

p₀ = 0.861 p₁ = 0.080 (p_2a + p_2b = 0.058)

2

<  0.0001 (ModelA vs M1)

Prob > 0.6 (11 L, 54R^a, 66Q^b, 180 M^a, 188Q, 200A^a, 248P, 273R^b, 274I^a, 275 T, 298 V, 344 V, 367 K, 389S)

ω₂ = 152.141

a4

Model A

–

p₀ = 0.801 p₁ = 0.074 (p_2a + p_2b = 0.11)

2

0.4525 (ModelA vs M1)

Prob > 0.6 (122 V, 174A, 185 M, 220R, 268A)

ω2 = 1.000

a5

Model A

–

p₀ = 0.902 p₁ = 0.083 (p_2a + p_2b = 0.015)

2

0.0028 (ModelA vs M1)

Prob > 0.6(94H^a, 182A, 196H^a, 269E)

ω2 = 11.724

a6

Model A

–

p₀ = 0.915 p₁ = 0.085 (p_2a + p_2b = 0.000)

2

1.0000 (ModelA vs M1)

Prob > 0.6 (13H, 201H)

ω2 = 3.081

a7

Model A

–

p₀ = 0.915 p₁ = 0.085 (p_2a + p_2b = 0.000)

2

1.0000 (ModelA vs M1)

Prob > 0.6 (33 K, 56 L)

ω2 = 1.000