Fig. 8

Drought tolerance in transgenic soybean plants. (A-D) Drought tolerance was evaluated in transgenic soybean plants. Transgenic soybean plants with positive hairy roots were transferred to plastic pots containing a mixture of peat and vermiculite (1:1, v/v) and grown for 7 days. Water deprivation for 16 days was followed by re-watering for 3 days. The hairy roots phenotype is shown in Fig. 8D. Drought resistance of transgenic soybean plants was assayed by the capability to resume growth when returned to normal conditions after drought stress. Scale bar, 5 cm. (E) Relative transcript of GmNFYA5 was detected in three transgenic soybean lines. Transcript of GmNFYA5 in EV plants was set at 1.0, and the expression levels were normalized to that of GmCYP2. (F-K) Survival rate, RWC, ion leakage, MDA, ABA concentration and SWP in transgenic soybean lines. Data represent mean SD for three biological replicates (n = 18). Significant differences P < 0.05 are indicated by different letters above the columns