Fig. 5

Allele discrimination for the Amplifluor-like SNP marker KATU-B30. Fifty F₃ breeding lines from the segregating population Granal × Baisheshek were used. Parental genotypes are designated as: ♀ P₁, Granal, and ♂ P₂, Baisheshek. Relative Fluorescence Units (RFU) for fluorophores FAM and HEX were transformed automatically into genotyping of alleles 1 and 2, respectively, using a BioRad CFX96 Real-Time PCR Detection System Instrument