Fig. 3

Identification of the osnadk1 mutant and morphology of WT (cv. Dongjin) and osnadk1 plants. a The gene structure of OsNADK1 (not to scale). Solid boxes and lines indicate exons and introns, respectively. The arrow indicates the T-DNA insertion position, and the triangle represents the T-DNA insertion. “LP”, “BP” and “RP” are the positions of primers for identification. b Screening of the osnadk1 mutant by PCR. Lanes 1, 3, 5, 7 show amplicons generated using the left and right primers (LP and RP, respectively) described in Methods; lanes 2, 4, 6, 8 show amplicons generated using the right primer and T-DNA-border primer (BP) described in Methods. a-c, Plants grown from PFG_1A-07609.R seeds. c The OsNADK1 transcript levels in WT and osnadk1 plants detected by semi-quantitative RT-PCR. β-ACTIN was used as a control. Lanes 1 and 2, WT; lanes 3 and 4, osnadk1. d The expression levels of OsNADK1 in the mutant and WT tested by qRT-PCR. e and f Gross morphology and agronomic characteristics of WT and osnadk1 plants at the early filling stage of growth. Values represent the mean ± SD of eight replicates. The letters denote significant differences (p ≤ 0.05) according to t-test analysis