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Fig. 3 | BMC Plant Biology

Fig. 3

From: Novel molecular components involved in callose-mediated Arabidopsis defense against Salmonella enterica and Escherichia coli O157:H7

Fig. 3

EXO70H4 contributes to bacterium-induced callose deposits, but not ROS burst. a Callose deposits area was measured in each genotype after four- to five-week-old plants were syringe-infiltrated water as a mock control or with 1 × 108 CFU.mL− 1 of Pst DC3118, STm 14,028 s, or E. coli O157:H7. Results are shown as average of three to four biological replicate (n = 18 to 37 ± SE) and the experiment was repeated twice with similar results. Statistical difference between the means (wild type versus mutant plant) was calculated using the two-tailed Student’s t-test (** = p < 0.01; *** = p < 0.001; ns = non-significant). The pictures on the left are representative of each plant genotype per treatment. b Leaf discs of four- to five-week-old plants were incubated with control solution or 1 × 108 CFU.mL− 1 of boiled Pst DC3118, STm 14,028 s, or E. coli O157:H7. Results are shown as the average of luminescence per genotype in three independent biological replicates (n = 21 to 37 ± SE). Luminescence was recorded over 60 min using a Synergy™ H1 microplate reader and the Biotek Gen5 (Biotek) software. RLU = Relative Light Units

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