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Fig. 8 | BMC Plant Biology

Fig. 8

From: Genome wide identification and functional characterization of strawberry pectin methylesterases related to fruit softening

Fig. 8

FvPME38 and FvPME39 involved in fruit softening. a, FvPME38 and FvPME39 were cell wall localized as determined by GFP-FvPME38 and GFP-FvPME39 fusion protein in tobacco leaf epidermal cells. Plasma membrane was identified by FM4–64 staining. b, Phenotypes of fruits were agro-infiltrated with FvPME38 and FvPME39 overexpression and RNAi constructs, respectively. DAI, day after infiltration; RNAi, RNA interference; OE, overexpression. c, QRT-PCR analysis of transcript levels for FvPME genes in overexpression and RNAi fruits at three days after injection. Error bars represent SD of three independent replicates. Letter in figure indicates significant differences between groups (P < 0.05, one-way ANOVA, Tukey’s HSD post hoc test). d-e, Pectin content of cell wall (D) and fruit size (E) at seven days after injection. Error bars represent SD of 15 fruits. f, Sections of overexpression and RNAi fruits at seven days after injection. g, QRT-PCR analysis of transcript levels for ripening-related genes in fruits of transient overexpression or silencing of FvPMEs. Relative expression levels of each gene were normalized to internal control Fvactin. Error bars represent SD of three independent replicates. PL, pectate lyase; PG, polygalacturonase; CEL, cellulose; XYL1, beta-xylosidase1; EXP1, expansin1; CHS, chalcone synthase; CHI, chalconeisomerase; F3H, flavanone 3-hydroxylase; DFR, dihydroflavonol 4-reductase; UFGT, UDP-glucose flavonoid 3-O-glycosyltransferase; ANS, anthocyanidin synthase; SS, sucrose synthase; SUT1, sucrose transporter1; SPS3, sucrose phosphate synthase3; HXK2, hexokinase2. h, Texture analysis of fruits were agro-infiltrated with FvPME38 and FvPME39 overexpression and RNAi constructs, respectively. i-j, Firmness value of overexpression and RNAi fruits at same ripening stages after infiltration. SR stage (I); FR stage (J). The number in bracket of x-axis labels represents the number of days after injection. Error bars represent SD of 15 fruits. Letter in figure indicates significant differences between groups (P < 0.05, one-way ANOVA, Tukey’s HSD post hoc test)

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