Table 6 Percentage (±SE) of explants with shoots and number of acclimatized plants from the experiment 3 for the accessions of common eggplant IVIA371, BB, and MM1597, and for S. insanum INS1 in two different tissues (cotyledon and hypocotyl) using 16 h light / 8 h dark photoperiod conditions and the E6 medium for organogenesis and the medium R2 for root formation induction. The percentage (±SE) of the diploid (2x) and tetraploid (4x) regenerated plants in experiment 4 are also reported. The number of initial explants used for each accession was n = 45
Accessions | Explants with shoots (%) | Acclimatized plants | Acclimatized plants / initial explants (%) | 2x regenerants (%) | 4x regenerants (%) |
|---|---|---|---|---|---|
Cotyledon | |||||
IVIA371 | 96.66 ± 0.03 | 36 | 80.00 ± 0.06 | 50.00 ± 0.08 | 50.00 ± 0.08 |
BB | 100.00 ± 0.00 | 15 | 33.33 ± 0.07 | 61.30 ± 0.13 | 38.70 ± 0.13 |
MM1597 | 69.23 ± 0.07 | 13 | 28.88 ± 0.06 | 64.71 ± 0.13 | 35.29 ± 0.13 |
INS1 | 100.00 ± 0.00 | 24 | 53.33 ± 0.07 | 75.00 ± 0.09 | 25.00 ± 0.09 |
Hypocotyl | |||||
IVIA371 | 86.66 ± 0.06 | 13 | 28.88 ± 0.06 | 65.00 ± 0.13 | 35.00 ± 0.13 |
BB | 70.00 ± 0.08 | 21 | 46.76 ± 0.07 | 66.70 ± 0.10 | 33.30 ± 0.10 |
MM1597 | 86.66 ± 0.06 | 9 | 20.00 ± 0.06 | 76.92 ± 0.14 | 23.08 ± 0.14 |
INS1 | 90.00 ± 0.05 | 17 | 37.77 ± 0.07 | 74.80 ± 0.11 | 25.20 ± 0.11 |