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Fig. 1 | BMC Plant Biology

Fig. 1

From: Characterization of a vacuolar sucrose transporter, HbSUT5, from Hevea brasiliensis: involvement in latex production through regulation of intracellular sucrose transport in the bark and laticifers

Fig. 1

HbSUT5 as a single or low copy vacuolar SUT gene. a. Phylogenetic tree of HbSUT5 and some selected plant sucrose transporters. Full length amino acid sequences of SUTs were exploited to create the phylogenetic tree by MEGA4. The GenBank accession numbers for the peptide sequences of other SUTs are At1g71880 (AtSUC1), At1g22710 (AtSUC2), At2g02860 (AtSUC3), At1g09960 (AtSUC4), At1g71890 (AtSUC5), At5g43610 (AtSUC6), At1g66570 (AtSUC7), At2g14670 (AtSUC8), At5g06170 (AtSUC9), BAA24071 (OsSUT1), BAC67163 (OsSUT2), BAB68368 (OsSUT3), BAC67164 (OsSUT4), BAC67165 (OsSUT5), ADW94615 (PtaSUT1), ADW94616 (PtaSUT3), ADW94617 (PtaSUT4), ADW94618 (PtaSUT5), ADW94619 (PtaSUT6), CAD61275 (LjSUT4), CAB75881 (HvSUT2), ABJ51933 (HbSUT1), ABJ51934 (HbSUT2A), ABJ51932 (HbSUT2B), ABK60190 (HbSUT3), ABK60191(HbSUT4) and ABK60189 (HbSUT5). b-e. Localization of HbSUT5-GFP and OsTIP1;1-RFP in rice protoplasts (Scale bar = 5 μm). b. Bright field image; c. Transient expression of GFP; d. Transient expression of RFP; e. Merged GFP and RFP image. f. DNA gel blot analysis of the HbSUT5 under high stringency. Genomic DNA from Hevea was digested with restriction analysis, resolved on a 0.8% agarose gel (20 μg/lane), and blotted to Hybond N+, and hybridized with a 32P-labled 528-bp-long HbSUT5 cDNA fragment

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