Table 2 Sequences of wheat SSR and EST-SSR markers
Marker | Primer sequence (5′-3′) | Annealing temperature (°C) | Arm location | ID of ESTs | References |
|---|---|---|---|---|---|
Xwmc14 | F: ACCCGTCACCGGTTTATGGATG R: TCCACTTCAAGATGGAGGGCAG | 63 | 7DL(terminal) | – | Somers et al. 2004 |
Xbarc111 | F: GCGGTCACCAGTAGTTCAACA R: GCGTATCCCATTGCTCTTCTTCACTAAC | 60 | 7DL (proximal) | – | Somers et al. 2004 |
Xcfd211 | F: AGAAGACTGCACGCAAGGAT R: TGCACTAAAGCATCTTCGTGTT | 65 | 3DL (terminal) | – | Guyomarc’h et al. 2002 |
Xcfd64 | F: ACAGTGTTGTTGCCCCTTTC R: CCCATGTTACAGCTTTGGGT | 64 | 3DS (terminal) | – | Guyomarc’h et al. 2002 |
1EST-255 | F: CCAGGACAGCCTATCCAAGA R: TCGAAGTTGGACTTCAGCAA | 57 | 1AL 1BL 1DL | Ta#S16058339 | Kong et al. 2018 |
1EST-1134 | F: CACAAACTATCCAAAGGATGA R: GTGGAACATTTTCAGGTGAC | 55 | 1AS 1DS | BG605065 | Kong et al. 2018 |
2EST-983 | F: ACAGGAGGTTGGATGAGTGG R: TCCACGTGTGTTTCGTCAAT | 57 | 2AS 2BS 2DS | BG314234 | Kong et al. 2018 |
2EST-705 | F: AGGTCACTGCAGGAGGAGGA R: GAAAAGATGATGAGCTGGTCTGG | 55 | 2BL 2DL | BF293175 | Kong et al. 2018 |
3EST-186 | F: CAATTTGTTGCCTACGTCA R: AGTTCTAATGGTGACCCACA | 55 | 3AL 3BL 3DL | BE406551 | Kong et al. 2018 |
3EST-147 | F: AAGCTCGTCTTCATCGTCTA R: GTACAGCCCCAGCAGGTA | 55 | 3AS 3BS 3DS | BF293133 | Kong et al. 2018 |
4EST-100 | F: GTGCACTCCGTCGAAGCTA R: AGGAGCTGGTGATGAACTGG | 58 | 4AS 4BL 4DL | BE497134 | Kong et al. 2018 |
4EST-19 | F: GTACGTAGCAGCCGATGGAT R: CCCCGATCGAGAAGTTACAA | 57 | 4AL 4DL | BE637934 | Kong et al. 2018 |
5EST-79 | F: AAGTATGCAGCCAGATCTCA R: GGTTATTGCTCTTGCAGATG | 54 | 5AL 5BL 5DL | BQ280540 | Kong et al. 2018 |
5EST-10 | F: GAGCTGGATCTTCAGCCTAT R: AATTTTTGCCATGAGATCG | 53 | 5AL | BM137728 | Kong et al. 2018 |
6EST-358 | F: GTACCATTCGATTGTTCTGC R: GGAAATCCTATGCCCTTAAT | 55 | 6AL 6BL 6DL | BE399146 | Kong et al. 2018 |
CINAU15 | F: AGATCCAACACCAGTTCAAG R: ATGTTATGGAGGCTTGTGTC | 53 | 6AS 6BS 6DS | Contig17515 | Kong et al. 2018 |
7EST-133 | F: CTCTTCCCCTCTCTCGTCCT R: GCTCCAAATCTTCACCAAGC | 57 | 7DS | Ta#S13057851 | Kong et al. 2018 |
7EST-138 | F: GATTAGGCAAATGGGTCA R: CTCATCGGGTTCAGTGGT | 55 | 7AS 7BS 7DS | BE494425 | Kong et al. 2018 |