Fig. 3

Relative expression of the candidate unigenes involved in isoflavone biosynthesis across four tissues of P. mirifica by qRT-PCR. Tissue abbreviations: Y; young leaves, M; mature leaves, T; cortex-excised tubers, C; tuberous cortices. Gene abbreviations: PAL; Phenylalanine ammonia-lyase, C4H; Cinnamate 4-monooxygenase, 4CL; Coumarate-CoA ligase, CHS; Chalcone synthase, CHI; Chalcone isomerase, IFS; 2-hydroxyisoflavone synthase, HID. Significantly different expression levels of putative genes (ANOVA, post-hoc Duncan test, P < 0.05) have different lowercase letters