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Fig. 7 | BMC Plant Biology

Fig. 7

From: Transcriptional induction of capsidiol synthesis genes by wounding can promote pathogen signal-induced capsidiol synthesis

Fig. 7

Wounding enhances subsequent induction of EAS protein and capsidiol by INF1: a Leaves of the wild type tobacco were wounded or infiltrated with 25 nM INF1, and harvested at the times indicated. The accumulation of EAS protein was investigated by immunoblotting analysis using an anti-EAS antibody (α-EAS). As a loading control, parallel gels were stained with Coomassie Brilliant Blue R-250 (CBB). b, c Leaves were infiltrated with 25 nM INF1 directly or at 9 h after being wounded by holding the leaves with forceps. The samples were harvested at the times indicated after INF1 treatment, and the accumulation of EAS protein (b) and capsidiol (c) was investigated by immunoblotting analysis using an anti-EAS antibody (α-EAS) and HPLC, respectively. b As a loading control, parallel gels were stained with Coomassie Brilliant Blue R-250 (CBB). c Values are means with standard errors of five or six biological replicates. Significant differences between not-wounded and pre-wounded were determined by Student’s t-test using Excel 2013 software (**P < 0.01)

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