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Fig. 6 | BMC Plant Biology

Fig. 6

From: Transcriptional induction of capsidiol synthesis genes by wounding can promote pathogen signal-induced capsidiol synthesis

Fig. 6

Multiple regions of the EAS4 promoter are involved in activation by WIPK and SIPK: Agrobacterium cells containing GUS driven by EAS4 promoter fragments was mixed with those carrying LUC driven by a 35S promoter and those expressing MEK2DD driven by a 35S promoter, and then infiltrated into the leaves of N. benthamiana. After incubation at 25 °C for 48 h, total RNA was extracted, and the transcript levels of GUS, LUC, and Nbactin2 were quantified by RT-qPCR. The levels of GUS were doubly normalized to the levels of Nbactin2 and LUC as internal and infection standards, respectively. a 5′-Deletion analysis of the EAS4 promoter. Values are means with standard deviations of three to six biological replicates. b Gain-of-function analysis of the EAS4 promoter. Four tandem repeats of the respective regions of the EAS4 promoter were fused to a 35S minimal promoter. Values are means with standard deviations of three biological replicates. Significant differences between Vector and MEK2DD were determined by Student’s t-test using Excel 2013 software (**P < 0.01, *P < 0.05)

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