Fig. 2

WIPK and SIPK are involved in wound-induced expression of the capsidiol synthesis genes: a, b Leaves of the vector control (V1) and WIPK/SIPK-suppressed plants (WS3) were wounded, and harvested at the times indicated after wounding. Transcript levels of EAS, EAH, and SQS (a), and genes of the mevalonate pathway (b) were quantified by RT-qPCR and normalized to the level of Actin2 as an internal standard. Values are means with standard deviations of three biological replicates. Significant differences among the groups were determined with one-way ANOVA followed by Tukey’s HSD using KaleidaGraph 4.5 software. The lowercase letters at the top of the bars indicate significant differences (P < 0.05)