Fig. 2

Identification of the d2014 gene. a Positional cloning of the d2014 gene: Firstly, the d2014 locus was mapped between the markers umc1281 and umc1278 on the chromosome 1 using 300 dwarf individuals from (d2014 × HL5054) × d2014 BC1 population. Secondly, we enlarged the population and developed new Indel molecular markers. The location of the d2014 gene was then narrowed down to a smaller segment flanked by the markers b4 and b15. Finally, using 2000 recessive plants, we locate the candidate region in about 470 Kb between the markers c5 and c7. The numbers below the vertical lines indicate the recombinants. b The BR2 and other six predicted genes in the 470 Kb region, and and the sequence alignment of BR2 in d2014 and WT. c Variation of the BR2 protein sequence between d2014 and WT