Fig. 5

Genes putatively involved in xylem cell differentiation display reduced expression in Mtnoot1 roots. a, b Expression level of various genes putatively involved in xylem and phloem cell differentiation in zone 3 and zone 4 of wild-type and Mtnoot1 tnk507 roots determined by qRT-PCR. a The MYB-type transcription factors MtMYB46–1, MtMYB46–2, MtMYB83 and MtMYB85, and the XYLYME CYSTEINE PEPTIDASE 1 putative ortholog MtXCP1 are lower expressed in zone 3 of Mtnoot1 mutant roots when compared to wild-type. c The expression level of phloem marker gene MtAPL is not affected in Mtnoot1 mutant roots. The data represent means + SEM of two (MtXCP1 and MtAPL) or three (MtMYBs) independent experiments. Student t-test was performed to assess significant differences (*: P < 0.05, ****: P < 0.0001, ns: not significant)