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Fig. 1 | BMC Plant Biology

Fig. 1

From: The Medicago truncatula nodule identity gene MtNOOT1 is required for coordinated apical-basal development of the root

Fig. 1

The primary root of the medicago Mtnoot1 mutant is longer than wild-type root. a Schematic representation of the zonation in a medicago primary root. Left panel: the size of the root apical meristem (RAM) is determined from the stem cell niche till the elongation/differentiation zone (EDZ). The apical border of the EDZ is defined by the first elongated cortex cell of second cortical layer, as indicated by arrowhead. Note that the exact position of the RAM-EDZ boundary can vary depending on the cell type, marking a region that named transition zone (TZ) [30, 31]. Cell types are indicated by colour coding. Right panel: Normaski image of the distal region of the RAM and apical region of the EDZ of a primary root at 6 Days After Germination (DAG). Arrowhead indicates the boundary between the RAM and the EDZ of the second layer of cortical cells. b Root length of the medicago Mtnoot1 tnk507 mutant is markedly longer at 4 DAG and 6 DAG when compared to primary roots of wild-type medicago seedlings (WT). c The RAM of medicago Mtnoot1 tnk507 seedlings is larger at 6 DAG when compared to the RAM of primary roots of wild-type medicago seedlings. The data represent means + SD of three independent experiments, each experiment contains 16–20 roots. d The cortical cell size at the proximal part of the RAM is not affected in the root of the medicago Mtnoot1 tnk507 mutant seedlings. Given is the average cell size of cortical cells at the proximal part of the RAM of wild-type and Mtnoot1 mutant roots. The data represent means + SD of two independent experiments, each experiment contains 16–20 roots. Student t-test was performed to assess significant differences (****: P < 0.0001, ns: not significant). A representation of the RAM of wild-type (e) and Mtnoot1 mutant seedling (f) primary roots at 6 DAG. White dotted line marks the quiescent center/stem cell niche region, arrowhead marks the boundary between the RAM and the EDZ of the second layer of cortical cells. Scale bar: 50 μm (a), 100 μm (e, f)

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