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Fig. 8 | BMC Plant Biology

Fig. 8

From: Comparative physiological, metabolomic, and transcriptomic analyses reveal developmental stage-dependent effects of cluster bagging on phenolic metabolism in Cabernet Sauvignon grape berries

Fig. 8

Molecular characterization and functional analysis of VviFLS4. (a) HPLC-MS analysis of reaction products generated from the incubation of VviFLS4-His recombinant protein with the substrates naringenin (N), dihydrokaempferol (DHK), dihydroquercetin (DHQ), and dihydromyricetin (DHM). K: kaempferol; Q: quercetin; M: myricetin. (b) Quantitative analysis of VviFLS4 gene expression in the skin of detached grape berries under UV-B radiation treatments. Each square in the heatmap corresponds to the relative expression of the gene as illustrated in the legend. Significant expression changes of VviFLS4 gene after treatment compared are indicated by asterisks (*) in the squares. (c) Schematic representation of the cis-acting elements predicted in the promoter region of the VviFLS4 gene. The promoter region and 5′-untranslated region are differentiated by grey and white boxes, respectively. The CAAT-boxes are indicated by red triangles. Values above boxes represent the number of nucleotides, starting from the putative transcription start site indicated by the blue triangle. CDS, coding sequence. (d) Transient expression analysis of VviFLS4 gene promoter activity in tobacco leaves. An equal amount of pVviFLS4-LUC construct was transformed into the left and right sides of tobacco leaves. The right sides of the infiltrated leaves were shaded with masking for dark shading treatments. Left panel: Schematic diagram of the leaf shading treatment. Middle panel: Fluorescence imaging. Right panel: Relative transcription level of the LUC gene in the experimental and control groups (** indicates significance at the 0.01 level)

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