Fig. 4

Confocal observation of red fluorescence in 35S::AtPDF2.1-mRFP transgenic A. thaliana. Subcellular localization merge map. Surface sterile seeds were plated onto 1/2 Murashige and Skoog medium and grown vertically for 2 weeks. The root epidermal cells were incubated in 40% sucrose to induce plasmolysis, and then imaged by confocal microscopy. The arrow points to the plasmic wall separation