Fig. 3

Expression patterns of AtPDF2.1. AtPDF2.1 is mainly expressed in shoot and root vascular bundles. a Analysis of the relative expression level of AtPDF2.1 in different organs of A. thaliana by quantitative real-time PCR. Tissues were harvested either from 45-d-old plants grown in hydroponics (leaves, stems, siliques, and flowers) or from 7-d-old plants grown in 1/2 MS medium (seedlings). Actin2 was used as an internal control. Data are mean ± SD, n = 4. b–e Histochemical localization of GUS activity in transgenic plants expressing the GUS reporter gene under the control of the proAtPDF2.1 promoter. b One-week-old whole-mount seedling root. c, d 45-d-old plants shoot (c) and leaves (d). e Cross-section of one-week-old root. Bars = 4 mm in (b), 2 cm in (c), 1 cm in (d), and 10 μm in (e)