Fig. 5From: The in vivo impact of MsLAC1, a Miscanthus laccase isoform, on lignification and lignin composition contrasts with its in vitro substrate preferenceLocalization of MsLAC1 in the cell wall compartment after transient expression in Nicotiana benthamiana leaf. Transient expression of MsLAC1-mCherry and LT16b-GFP (plasma membrane marker fused with GFP) in tobacco epidermal leaf cells after co-infiltration with Agrobacteria harboring the respective constructs. Upper panel shows cell wall association of MsLAC1, whereas the Lti6-GFP-labelled plasma membrane retracts. Lower panel shows a cell co-expressing Lti6b-GFP and a cytosolic mCherry control retracting with the protoplast. All pictures were taken 2 h after onset of plasmolysis (i.e. incubation in 30% w/v sucrose solution). Scale bars: 16 μmBack to article page