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Fig. 2 | BMC Plant Biology

Fig. 2

From: A T-DNA mutant screen that combines high-throughput phenotyping with the efficient identification of mutated genes by targeted genome sequencing

Fig. 2

Detection of NO2-sensitive and -tolerant mutants. a. Approximately 3000 mutant plants per tray were grown for 2 weeks. Plants were fumigated for 1 h with 10 ppm NO2 and 2 days later with 30 ppm NO2. b Example of a sensitive mutant showing lesions after fumigation with 10 ppm NO2 while neighbouring plants were unaffected. Pictures were taken at 48 h after fumigation. c Most plants were severely damaged after exposure to 30 ppm NO2. d However, tolerant mutants exhibited only weak symptoms. Pictures were taken at 48 h – 96 h after fumigation. Note that the highlighted (red arrows) mutants exhibited altered NO2-induced symptoms compared to all neighboring plants

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BMC Plant Biology

ISSN: 1471-2229

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