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Fig. 4 | BMC Plant Biology

Fig. 4

From: Overexpression of TCP8 delays Arabidopsis flowering through a FLOWERING LOCUS C-dependent pathway

Fig. 4

FLC is required for the TCP8 function in regulating flowering time. a. Relative transcription levels of TCP8 in flc-6 and 35S::TCP8/flc-6 detected by RT-qPCR. Data are represented as mean ± S.D. of three biological replicates. ACTIN2 was used as the endogenous control for normalizing the transcription levels of TCP8. The transcription level of TCP8 in flc-6 was arbitrarily set to 1. b. The number of rosette leaves in flc-6 and 35S::TCP8/flc-6 plants before bolting. c. Forty-day-old flc-6 and 35S::TCP8/flc-6 plants grown under LD condition. d. Transcription levels of TCP8 in wild-type Col-0, 35S::TCP8, flc-6 and 35S::TCP8/flc-6 individuals. ACTIN2 was used as the endogenous control for data normalization and the transcription level of TCP8 is represented as mean ± S.D. of three biological replicates. The transcription level of TCP8 in Col-0 was arbitrarily set to 1. e. The number of rosette leaves in wild-type Col-0, 35S::TCP8, flc-6 and 35S::TCP8/flc-6 before bolting. (Student’s t-test, *** P < 0.001, ns = not significant). f. Forty-day-old wild-type Col-0, 35S::TCP8, flc-6 and 35S::TCP8/flc-6 plants grown under LD condition

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